siRNA / miRNA gene silencing Rat Brain endothelial cells HIF-1α

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Rat Hippocampus

Get tips on using NucleoSpin® RNA/Protein to perform RNA isolation / purification Tissue - Rat Hippocampus

Get tips on using MMP-3 (D7F5B) Rabbit mAb #14351 to perform Immunohistochemistry Rat - MMP3

Get tips on using DNA Isolation Kit for Cells and Tissues to perform DNA isolation / purification Cells - Primary cells Human primary keratinocytes

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Liver

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Kidney

Get tips on using Anti-trimethyl-Histone H3 (Lys9) Antibody to perform ChIP H3K9me3 - Sheep Rat -NA-

Get tips on using Anti-trimethyl-Histone H3 (Lys4) Antibody to perform ChIP H3K4Me3 - Sheep Rat -NA-

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Cerebellum

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.