DNA isolation / purification Cells Primary cells

Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - BHK-21

Get tips on using CytoBuster™ Protein Extraction Reagent to perform Protein isolation Mammalian cells - CHO-K1

Get tips on using BisulFlash DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - A2780 TIMP2

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - SKOV3 H19

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - SKOV3 PEG1

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Whole genome profiling - mouse iPSCs

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - HEK 293T RBP3

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - Caco-1 CHST11