Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Get tips on using pANC233 (xylG) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylG
Get tips on using pANC232 (xylF) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylF
Get tips on using pANC231 (xylE) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylE
Get tips on using pANC230 (xylD) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylD
Get tips on using pANC223 (xylC) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylC
Get tips on using pANC210 (xylB) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylB
Get tips on using pANC209 (xylA) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylA
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment