DNA transfection Mammalian cells Immortalized cell lines

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Cells - Primary cells HUVEC

Get tips on using CytoTox 96® Non-Radioactive Cytotoxicity Assay to perform Live / Dead assay mammalian cells - CHO-K1

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Get tips on using illustra tissue and cells genomicPrep Mini Spin Kit to perform DNA isolation / purification Tissue - kidney

Get tips on using Leibovitz's L-15 Medium to perform Mammalian cell culture media MDA-MB-468

Get tips on using Gibco™ RPMI 1640 Medium to perform Mammalian cell culture media HL-60

Get tips on using Gibco™ RPMI 1640 Medium to perform Mammalian cell culture media MCF-7