Get tips on using Micro BCA™ Protein Assay Kit to perform Protein quantification Mammalian cells - Human pluripotent stem cells
Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - Human pluripotent stem cells
Get tips on using CD163 Antibody, anti-human, PE-Vio® 770, REAfinity™ to perform Flow cytometry Anti-bodies Human - CD163
Get tips on using Alexa Fluor® 488 anti-human CD127 (IL-7Rα) Antibody to perform Flow cytometry Anti-bodies Human - CD127
Get tips on using Alexa Fluor® 488 anti-human CD15 (SSEA-1) Antibody to perform Flow cytometry Anti-bodies Human - CD15
Get tips on using Human/Mouse/Rat Activin A Quantikine ELISA Kit to perform ELISA Rat - Activin
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,
Get tips on using Monoclonal Mouse Anti-Human p53 Protein (Dako Omnis) Clone DO-7 to perform Immunohistochemistry Human - p53
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment