siRNA / miRNA gene silencing Human Lymphoma cell line U937

Get tips on using Human Thrombopoietin R/Tpo R APC-conjugated Antibody to perform Flow cytometry Anti-bodies Human - CD110/Thrombopoietin R

Get tips on using Human Thrombopoietin R/Tpo R PE-conjugated Antibody to perform Flow cytometry Anti-bodies Human - CD110/Thrombopoietin R

Get tips on using Human IL-3R alpha /CD123 PE-conjugated Antibody to perform Flow cytometry Anti-bodies Human - CD123/IL3-R

Get tips on using PE-Cy™7 Mouse Anti-Human CD123 to perform Flow cytometry Anti-bodies Human - CD123/IL3-R

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.