Cell Isolation B cell

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Found 10 matching solutions for this experiment

Protocol tips
purified B cells (>95%) were isolated using MagCellect Human B Cell Isolation Kit (R&D systems, USA). B cell purity was assessed by flow cytometry using APC labeled CD19 antibody (Biolegend, USA).
Protocol tips
During positive selection, the cell type of interest (in this case B-cells) are specifically targeted, labelled and actively magnetically removed from the sample by retention in the column in the presence of a magnetic field. These cells are then retrieved by plunging the column in the absence of a magnetic field.
Protocol tips
B cells were purified from mouse spleen or human PBMC using magnetic negative selection kits (StemCell Technologies).
Protocol tips
HCL and HCLv cells for microarray studies were obtained in sodium heparin tubes and purified by Ficoll centrifugation, followed by total B-cell isolation using the Dynabeads™ Untouched™ Human B Cells Kit (ThermoFisher Scientific).
Protocol tips
CLL cells were negatively selected from fresh blood samples using the RosetteSepTM B cell enrichment cocktail (StemCell Technologies, Grenoble, France) and density gradient centrifugation (Ficoll®Paque Plus, GE Healthcare Life sciences, Velizy-Villacoublay, France).
Protocol tips
Human CD43− resting B cells were isolated from PBMCs using MojoSort Human B Cell (CD43−) Isolation Kit (BioLegend), according to the manufacturer's protocols.
Downstream tips
Isolated resting B cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, 1 mM sodium pyruvate, 10 mM HEPES, 100 μg/ml streptomycin, 100 U/ml penicillin, and 1 mM 2-mercaptoethanol.
Upstream tips
For positive selection, an aliquot of 10×106 frozen PBMCs was used for sequential isolations of B cells followed by CD8+ T cells and another 10x106 frozen PBMC aliquot was used for sequential isolation of monocytes followed by CD4+ T cells.
Protocol tips
according to manufacturer's instructions.
Upstream tips
For positive selection, an aliquot of 10×106 frozen PBMCs was used for sequential isolations of B cells followed by CD8+ T cells and another 10x106 frozen PBMC aliquot was used for sequential isolation of monocytes followed by CD4+ T cells.
Protocol tips
according to manufacturer's instructions.
Upstream tips
Protocol tips
Following this B cells were enriched by magnetic microparticle purification using the EasySep Human B cell Enrichment Kit Without CD43 Depletion.
Upstream tips
Protocol tips
Primary B-cells were purified from peripheral blood using EasySep™ Direct Human B Cell Isolation Kit from Stemcells Technologies Cat #19674 and directly used for RNA purification.
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