Get tips on using pMSCV-LTR-dCas9-VP64-BFP to perform CRISPR Mouse - Activation Neuro-2a Sim1
Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Mouse - Activation 3T3-L1 eIF5A1
Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Mouse - Activation 3T3-L1 ZPR9
Get tips on using Q5® Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Mouse - Deletion 3T3-L1 TMEM120A-SBP
Get tips on using pSpCas9(BB)-2A-Puro (PX459) V2.0 to perform CRISPR Mouse - Activation 3T3-L1 Adrb3
Get tips on using FITC Mouse Anti-Mouse NK-1.1 to perform Flow cytometry Anti-bodies Mouse - NK1.1
Get tips on using APC Mouse Anti-Mouse NK-1.1 to perform Flow cytometry Anti-bodies Mouse - NK1.1
Get tips on using Alexa Fluor® 700 Mouse Anti-Mouse NK1.1 to perform Flow cytometry Anti-bodies Mouse - NK1.1
Get tips on using Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) to perform Flow cytometry Anti-bodies Mouse - CD16/CD32
Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.
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