CRISPR Rat Deletion AR42J

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - AR42J

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - AR42J

Get tips on using MAP LC3β Antibody (G-2) to perform Autophagy assay cell type - AR42J

Get tips on using Micro BCA™ Protein Assay Kit to perform Protein quantification Mammalian cells - AR42J

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - AR42J

Get tips on using Phusion Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Rat - Deletion H9C2 SRF

Get tips on using Rat SCF ELISA to perform ELISA Rat - SC

Get tips on using Prolactin Rat ELISA to perform ELISA Rat - PRL

Get tips on using Rat Gastrin EIA to perform ELISA Rat - Gastrin

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.