Get tips on using Anti-CTCF Antibody to perform ChIP Anti-bodies CTCF
Get tips on using Anti-Ctip1/BCL-11A antibody [14B5] (ab19487) to perform ChIP Anti-bodies CtIP/BCL11A
Get tips on using Histone H2A.X antibody (pAb) to perform ChIP Anti-bodies γH2AX
Get tips on using Histone H4K20me1 antibody (mAb) to perform ChIP Anti-bodies H4K20me1
Get tips on using Histone H3K27ac antibody (pAb) to perform ChIP Anti-bodies H3K27ac
ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.
Get tips on using Bcl-11B (D6F1) XP® Rabbit mAb #12120 to perform ChIP Anti-bodies CtIP/BCL11A
Get tips on using ChromaFlash Chromatin Extraction Kit to perform ChIP Rat - Heart
Get tips on using ChromaFlash Chromatin Extraction Kit to perform ChIP Mouse - Osteoblasts
Get tips on using PARP-1 C-terminal antibody (pAb) to perform ChIP Anti-bodies PARP
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