Flowcytometry Secondary Antibody Goat Mouse

Get tips on using Anti-Ki-67 Antibody to perform Immunohistochemistry Ki67 - Rabbit Mouse -NA-

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Anti-Collagen X antibody (ab58632) to perform Immunohistochemistry Mouse - Col X

Get tips on using Anti-Collagen II antibody (ab34712) to perform Immunohistochemistry Mouse - Col II

Get tips on using Anti-EpCAM antibody [323/A3] (ab85987) to perform Immunohistochemistry Mouse - EpCAM

Get tips on using PLZF Antibody (D-9): sc-28319 to perform Immunohistochemistry Mouse - PLZF

Get tips on using Anti-ZBTB16 antibody produced in rabbit to perform Immunohistochemistry Mouse - PLZF

Get tips on using Neuron specific enolase monoclonal antibody (47) to perform Immunohistochemistry Mouse - NSE

Get tips on using CD24 Antibody (M1/69): sc-19651 to perform Immunohistochemistry Mouse - CD24

Get tips on using Recombinant Anti-CD34 antibody [EP373Y] (ab81289) to perform Immunohistochemistry Mouse - CD34