siRNA / miRNA gene silencing Human HeLa EPAS-1

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Oris™ Cell Migration Assay to perform Wound healing assay cell type - human MCF-10A

Get tips on using IncuCyte® Cell Migration Kit to perform Wound healing assay cell type - human MCF-10A

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Human Kidney

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Human Liver

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Human Veins

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Human Tonsil

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Human Prostate

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Human Muscles