Protein expression and purification

Get tips on using Trichloroacetic acid to perform Protein isolation Bacteria - Chlamydia pneumoniae

Get tips on using Trichloroacetic acid to perform Protein isolation Bacteria - Bacillus subtilis

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - Rat_Liver

Get tips on using Qproteome Mitochondria Isolation Kit to perform Protein enrichment Mitochondria

Get tips on using Ni-NTA HisSorb Strips (24) to perform Protein tag Detection of His-tagged proteins

Get tips on using Ni-NTA Spin Kit (50) to perform Protein tag Detection of His-tagged proteins

Get tips on using Penta·His Alexa Fluor 647 Conjugate to perform Protein tag Detection of His-tagged proteins

Get tips on using Penta·His Alexa Fluor 488 Conjugate to perform Protein tag Detection of His-tagged proteins

Get tips on using 3D-Gene® Mouse miRNA Oligo chip (ver.21) to perform Microarray Gene expression arrays - Mouse liver tissue Cyanine-3-CTP

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.