Site Directed Mutagenesis (SDM) Rat Point mutation Rat-2

Get tips on using siGENOME Human AP1G1 (164) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HeLa γ1-adaptin/AP1G1

Get tips on using siGENOME Human CHUK (1147) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - PANC-1 IKKα/CHUK

Get tips on using siGENOME Human IKBKB (3551) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - PANC-1 IKKβ/IKBKB

Get tips on using ON-TARGETplus Mouse Stat5b (20851) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - FL83B Stat5b

Get tips on using ON-TARGETplus Mouse Tead4 (21679) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - C2C12 Tead4

Get tips on using ON-TARGETplus Mouse Tead2 (21677) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - C2C12 Tead2

Get tips on using ON-TARGETplus Mouse Tead1 (21676) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - C2C12 Tead1

Get tips on using Accell Mouse Nrep (27528) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - 3T3-L1 P311/Nrep

Get tips on using ON-TARGETplus Human ELMOD2 (255520) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HeLa ELMOD2

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.