siRNA / miRNA gene silencing Human HeLa EPAS-1

- Found 6554 results

miRNeasy FFPE Kit Product

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - human kidney tissue

Products Qiagen miRNeasy FFPE Kit

miRNeasy Mini kit Product

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - human brain tissue

Products Qiagen miRNeasy Mini kit

miRNeasy Mini kit Product

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary human keratinocytes

Products Qiagen miRNeasy Mini kit

Some help with RNA isolation using Trizol Discussion

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Discussions Some help with RNA isolation using Trizol

CytoSelect™ 24-Well Wound Healing Assay Product

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human HMEC