DNA transfection Mammalian cells Immortalized cell lines

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When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary mouse tracheal epithelial cells

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary human airway epithelial cells

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary porcine tracheal epithelial cells

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

Discussions What DNA isolation kit would work for insect samples?

Get tips on using Gibco™ DMEM, high glucose to perform Mammalian cell culture media MDA-MB-231

Products Fisher Scientific Gibco™ DMEM, high glucose

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media MDA-MB-468

Products Thermo Fisher Scientific DMEM–Dulbecco's Modified Eagle Medium

DNA DNA isolation / purification Bacteria Gram negative Klebsiella

DNA DNA isolation / purification Bacteria Gram negative Rhodopseudomonas

DNA DNA isolation / purification Tissue genital / cervical samples

Get tips on using HyClone™ Dulbecco's Modified Eagles Medium to perform Mammalian cell culture media MCF-7

Products Cytiva HyClone™ Dulbecco's Modified Eagles Medium

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