Protein Expression Prokaryotic cells A. tumefaciens

Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Synechococcus elongatus

Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Salmonella enterica

Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Vibrio cholerae

Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Escherichia coli

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Mouse heart

Get tips on using Ni-NTA Superflow 96 BioRobot Kit (4) to perform Protein tag Purification of His-tagged proteins

Get tips on using Cell Lysis Buffer (10X) to perform Protein isolation Tissue - Mouse lung tissue

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using TRI Reagent™ Solution to perform Protein isolation Bacteria - Vibrio cholerae

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.