Get tips on using Subcellular Protein Fractionation Kit for Cultured Cells to perform Protein isolation Mammalian cells - SH-SY5Y
Plasmid isolation is an important technique in molecular biology or any kind of genetic editing. It involves amplifying plasmids overnight by transforming them into competent bacterial cells. The desired colonies of these bacteria can then be grown in shaker cultures, at appropriate shaking speed, oxygen availability and temperature. These liquid cultures can then be ultracentrifuged to pellet the bacteria, which are then used for plasmid isolation. The bacteria are first resuspended in a buffer, then lysed, neutralized, purified in a column, eluted, precipitated with ethanol and then resuspended. During plasmid isolation, it is important to lyse cells quickly because lysing bacteria for too long may lead to irreversible denaturing of the plasmid. Usually, alkaline lysis is used for isolation because it is a mild treatment. It isolates plasmid DNA and other cell components such as proteins by breaking cells apart with an alkaline solution. Precipitation removes the proteins, and the plasmid DNA recovers with alcohol precipitation. Resuspension and lysis buffers should be mixed thoroughly in order to prevent the DNA from breaking into smaller fragments. This is because broken gDNA can reanneal and remain in the solution, without binding to the column.
Get tips on using MEBMTM Mammary Epithelial Cell Growth Basal Medium to perform 3D Cell Culture Media Mouse primary breast cancer ephitelial cells-Mammospheres
Get tips on using Dynabeads™ Untouched™ Human T Cells Kit to perform Cell Isolation Human T cells
Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - Rat vascular smooth muscle cells (vSMCs)
Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - human trophoblast cells
Get tips on using AllPrep DNA/RNA/Protein Mini Kit to perform Protein isolation Tissue - Human placental tissue
Get tips on using Slc1a2 to perform siRNA / miRNA gene silencing Rat - Glial cells GLT-1
Get tips on using Gabpb1 siRNA to perform siRNA / miRNA gene silencing Rat - Schwann cells Nrf2
Get tips on using Ccr2 siRNA to perform siRNA / miRNA gene silencing Rat - Glial cells CCR2
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment