dna-quantification-human-hela

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression

Get tips on using HLA-DR Monoclonal Antibody (LN3), eBioscience™ to perform Flow cytometry Anti-bodies Human - HLA-DR

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat pulmonary artery smooth muscle cell (pPASMC)

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat pulmonary artery smooth muscle cell (pPASMC)

Get tips on using Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE to perform DNA isolation / purification Bacteria - Gram positive Lactobacillus

Get tips on using Hs_DNMT3B_2 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - HCT-116 DNMT3B

Get tips on using lenti dCAS-VP64_Blast to perform CRISPR Human - Activation interferon-γ promoter

Get tips on using pLV hUbC-dCas9-T2A-GFP to perform CRISPR Human - Repression HS2

Get tips on using pHR-SFFV-dCas9-BFP-KRAB to perform CRISPR Human - Repression CXCR4

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.