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Get tips on using RiboMinus™ Transcriptome Isolation Kit, bacteria to perform RNA isolation / purification Bacteria - Gram negative Pseudomonas aeruginosa

Products Thermo Fisher Scientific RiboMinus™ Transcriptome Isolation Kit, bacteria

Get tips on using MagNA Pure Compact RNA Isolation Kit to perform RNA isolation / purification Cells - primary porcine tracheal epithelial cells

Products Roche Lifesciences MagNA Pure Compact RNA Isolation Kit

Get tips on using SV 96 Total RNA Isolation System to perform RNA isolation / purification Cells - primary human airway epithelial cells

Products Promega SV 96 Total RNA Isolation System

Get tips on using MagNA Pure Compact RNA Isolation Kit to perform RNA isolation / purification Cells - primary human marrow stromal cells

Products Roche Lifesciences MagNA Pure Compact RNA Isolation Kit

Get tips on using MagNA Pure Compact RNA Isolation Kit to perform RNA isolation / purification Cells - primary human mesenchymal stem cells

Products Roche Lifesciences MagNA Pure Compact RNA Isolation Kit

Get tips on using TIANamp Genomic DNA Kit to perform DNA isolation / purification Cells - Primary cells Mouse embryonic fibroblast (MEF)

Products Tiangen TIANamp Genomic DNA Kit

Get tips on using Aurum™ Total RNA Mini Kit to perform RNA isolation / purification Cells - primary mouse cortical neurons

Products Bio-Rad Laboratories Aurum™ Total RNA Mini Kit

Get tips on using SV Total RNA Isolation System to perform

Products Promega SV Total RNA Isolation System

Get tips on using SV 96 Total RNA Isolation System to perform RNA isolation / purification Cells - primary porcine primary airway epithelial cell

Products Promega SV 96 Total RNA Isolation System

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

DNA Site Directed Mutagenesis (SDM) Mouse Point mutation 3T3-L1 S6 kinase 1

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