rna-isolation-purification-tissue-mouse-spinal-cord

Get tips on using Mouse SDF-1 α / CXCL12 α ELISA Kit to perform ELISA Mouse - SDF-1/CXCL12

Get tips on using Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit to perform ELISA Mouse - SDF-1/CXCL12

Get tips on using Mouse IL-1 Beta PicoKine™ ELISA Kit to perform ELISA Mouse - IL-1 beta

Get tips on using Mouse ICAM-1 / CD54 PicoKine™ ELISA Kit to perform ELISA Mouse - ICAM-1/CD54

Get tips on using Purified Rat Anti-Mouse CD24 Clone M1/69 (RUO) to perform Immunohistochemistry Mouse - CD24

Get tips on using Purified Rat Anti-Mouse CD24 Clone M1/69 (RUO) to perform Immunohistochemistry Mouse - CD24

Get tips on using Human/Mouse/Rat Total HSP70/HSPA1A DuoSet IC ELISA to perform ELISA Mouse - HSP70

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using MUC2 (MRQ-18) Mouse Monoclonal Antibody to perform Immunohistochemistry Human - Muc-2

Get tips on using Cyclin E1 (HE12) Mouse mAb #4129 to perform Western blotting Cyclin E