siRNA / RNAi /miRNA transfection Rat Ar4-2j (Pancreatic tumor)

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

Get tips on using EZ DNA Methylation-Direct Kit to perform DNA methylation profiling Gene specific profiling - A54 RASSF1A

Get tips on using Micro BCA™ Protein Assay Kit to perform Protein quantification Mammalian cells - AR42J

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - AR42J

Get tips on using TRIzol™ Plus RNA Purification Kit to perform RNA isolation / purification Cells - primary rabbit aortic endothelial cells

Plasmid isolation is an important technique in molecular biology or any kind of genetic editing. It involves amplifying plasmids overnight by transforming them into competent bacterial cells. The desired colonies of these bacteria can then be grown in shaker cultures, at appropriate shaking speed, oxygen availability and temperature. These liquid cultures can then be ultracentrifuged to pellet the bacteria, which are then used for plasmid isolation. The bacteria are first resuspended in a buffer, then lysed, neutralized, purified in a column, eluted, precipitated with ethanol and then resuspended. During plasmid isolation, it is important to lyse cells quickly because lysing bacteria for too long may lead to irreversible denaturing of the plasmid. Usually, alkaline lysis is used for isolation because it is a mild treatment. It isolates plasmid DNA and other cell components such as proteins by breaking cells apart with an alkaline solution. Precipitation removes the proteins, and the plasmid DNA recovers with alcohol precipitation. Resuspension and lysis buffers should be mixed thoroughly in order to prevent the DNA from breaking into smaller fragments. This is because broken gDNA can reanneal and remain in the solution, without binding to the column.

Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Mouse - RAW264.7

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized 293T

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized VCaP

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized U87