DNA transfection Mammalian cells

Get tips on using DNeasy PowerWater Kit (100) to perform DNA isolation / purification Water samples

Get tips on using DNeasy PowerBiofilm Kit (50) to perform DNA isolation / purification Biofilm samples

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - adipose

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - placenta

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - eye

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - lung

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - brain

Get tips on using DNeasy PowerLyzer PowerSoil Kit (100) to perform DNA isolation / purification Insects

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.