Get tips on using Agilent RNA 6000 Pico Kit to perform RNA quantification Fuorimetric - human saliva
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using Ion Total RNA-Seq Kit v2 to perform RNA sequencing Rat - PC12
Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Rat - PC12
Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Rat - A7R5
Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Mouse - RAW264.7
Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - mouse kidney tissue
Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - mouse liver tissue
Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - mouse adipose tissue
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment