siRNA / miRNA gene silencing Rat Schwann cells

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized Sf9

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized PNT2

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized HepG2

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized HaCaT

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized A2780

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized BEAS-2B

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized A2780/DDP

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary human keratinocytes

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.