Select a Cell type


DNA isolation / purification Bacteria

- Found 5258 results

SV Total RNA Isolation System Product

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Bacteria - Gram negative Helicobacter pylori

Products Promega SV Total RNA Isolation System
High Pure RNA Isolation Kit Product

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram negative Haemophilus influenzae

Products Roche Lifesciences High Pure RNA Isolation Kit
High Pure RNA Isolation Kit Product

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram negative Chlamydia pneumoniae

Products Roche Lifesciences High Pure RNA Isolation Kit
PureLink ™ miRNA Isolation Kit Product

Get tips on using PureLink ™ miRNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis

Products Thermo Fisher Scientific PureLink ™ miRNA Isolation Kit
High Pure RNA Isolation Kit Product

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram negative Vibro parahaemolyticus

Products Roche Lifesciences High Pure RNA Isolation Kit
High Pure RNA Isolation Kit Product

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram negative Neisseria gonorrhoeae

Products Roche Lifesciences High Pure RNA Isolation Kit
High Pure RNA Isolation Kit Product

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram positive Streptococcus pneumoniae

Products Roche Lifesciences High Pure RNA Isolation Kit
SV Total RNA Isolation System Product

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Bacteria - Gram positive Streptococcus pneumoniae

Products Promega SV Total RNA Isolation System
pET-21a(+) DNA Product

Get tips on using pET-21a(+) DNA to perform Protein expression and purification Bacteria - Escherichia coli Prefoldin (PFD)

Products Merck Millipore pET-21a(+) DNA
RNA isolation / purification Cells - primary human endometrial stromal cells Experiment

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

RNA RNA isolation / purification Cells primary human endometrial stromal cells

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms