DNA isolation / purification Tissue

Get tips on using PureLink Genomic DNA Mini Kit to perform DNA isolation / purification Tissue - eye

Get tips on using QIAamp DNA Blood Mini Kit to perform DNA isolation / purification Tissue - eye

Get tips on using Quick-DNA™ Miniprep Kit to perform DNA isolation / purification Tissue - adipose

Get tips on using AllPrep DNA/RNA Mini Kit to perform DNA isolation / purification Tissue - liver

Get tips on using Quick-DNA™ FFPE Kit to perform DNA isolation / purification Tissue - kidney

Get tips on using AllPrep DNA/RNA Mini Kit to perform DNA isolation / purification Tissue - colon

Get tips on using AllPrep DNA/RNA Mini Kit to perform DNA isolation / purification Tissue - spleen

Get tips on using illustra tissue and cells genomicPrep Mini Spin Kit to perform DNA isolation / purification Tissue - kidney

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.