DNA transfection Mammalian cells

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using DNeasy PowerSoil Pro Kit to perform DNA isolation / purification Yeast - Saccharomyces boulardii

Get tips on using DNeasy UltraClean Microbial Kit to perform DNA isolation / purification Yeast - Candida parapsilosis

Get tips on using DNeasy UltraClean Microbial Kit to perform DNA isolation / purification Yeast - Saccharomyces cerevisiae

Get tips on using DNeasy Plant Mini Kit (250) to perform DNA isolation / purification Plants - Fungus

Get tips on using DNeasy 96 Plant Kit (6) to perform DNA isolation / purification Plants - Leaves

Get tips on using DNeasy mericon Food Kit (50) to perform DNA isolation / purification Processed food

Get tips on using DNeasy PowerMax Soil Kit (10) to perform DNA isolation / purification Soil samples

Get tips on using DNeasy PowerWater Sterivex Kit (50) to perform DNA isolation / purification Water samples

Get tips on using Enzo BioArray™ Single-Round RNA Amplification and Biotin Labeling System to perform RNA amplification & labeling Mammalian - RNA amplification and Labeling Brain tissue from rhesus monkey Biotin