siRNA / miRNA gene silencing Human Human ovarian carcinoma cell (OV2008) Yap Gene

Get tips on using Click-iT™ Plus EdU Pacific Blue™ Flow Cytometry Assay Kit to perform Cell cycle assay human - SKOV3

Get tips on using DeadEnd™ Colorimetric TUNEL System to perform TUNEL assay cell type - HNSCC Detroit 562 human head and neck tumor cells

Get tips on using DMEM/F-12, no phenol red to perform 3D Cell Culture Media Human breast cancer MDA-MB-231 cells-Mammospheres

Get tips on using DMEM/F-12 PLUS Basal Medium to perform 3D Cell Culture Media Human breast cancer MDA-MB-231 cells-Mammospheres

Get tips on using Gibco™ DMEM, high glucose, GlutaMAX™ Supplement to perform Stem cell culture media Human Dental pulp stem cells (hDPSC)

Get tips on using QIA33 | FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme to perform Apoptosis assay cell type - Human endometrial stromal cells

Get tips on using CellROX™ Deep Red Reagent, for oxidative stress detection to perform ROS assay cell type - human umbelical vein endothelial cells (HUVEC)

Get tips on using Senescence Cells Histochemical Staining Kit to perform Reporter gene assay β-galactosidase substrates - adipose stem cells

Get tips on using Senescence Cells Histochemical Staining Kit to perform Reporter gene assay β-galactosidase substrates - mouse embryonic fibroblasts

The challenge in isolating RNA from S. aureus cells is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads is considered to be the better alternative.