Microarray Gene expression arrays Mouse liver tissue

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Breast

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Bone

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Rat - Gingival tissue

Get tips on using QIAamp Media MDx Kit (12) to perform DNA isolation / purification Tissue - genital / cervical samples

Get tips on using miScript II RT Kit (50) to perform cDNA synthesis Tissue

Get tips on using First-Strand cDNA Synthesis Kit to perform cDNA synthesis Tissue

Get tips on using ReadyScript® cDNA Synthesis Mix to perform cDNA synthesis Tissue

Get tips on using SuperScript® II Reverse Transcriptase to perform cDNA synthesis Tissue

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform Stem cell culture media Choroid plexus-like tissue generation from SFEBq