Microarray Gene expression arrays Rat pancreas tissue

Get tips on using Freedom™ DG44 Kit to perform Protein expression and purification Mammalian cells - CHO-K1 G12

Get tips on using pFBDM-PS1(wt)-γ-secretase to perform Protein Expression Eukaryotic cells - S. frugiperda γ-secretase

Get tips on using pGAPZA-α-MF-op-rLlAlp2 to perform Protein Expression Eukaryotic cells - P. pastoris Alkaline phytase

Get tips on using pTRAkc-rbcs1-cTP/pRIC 3.0 to perform Protein Expression Prokaryotic cells - A. tumefaciens BFDV cp

Get tips on using pIEX-4-Crypα-6His-mMBP-UMODpXR to perform Protein Expression Eukaryotic cells - S. frugiperda mMBP

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat pulmonary artery smooth muscle cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat dorsal root ganglion neurons

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat brain microvascular endothelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat aortic smooth muscle cells