shRNA gene silencing Human THP-1

Get tips on using QuikChange Site-Directed Mutagenesis Kit, 10 Rxn to perform Site Directed Mutagenesis (SDM) Human - Point mutation SKOV3 humanized trastuzumab

Bacterial culture is a process of letting bacteria multiply in a controlled fashion (temperature, humidity, oxygen content or shaking), in a predetermined culture medium (antibiotic resistance to obtain homogenous clones). It is an important step, especially during cloning, as a single cell can be grown homogeneously (on semi-solid or in liquid conditions) to obtain colonies. As mentioned, bacteria can be cultured in broth cultures (Luria broth or LB) or Petri dishes (Agar plates). A specific antibiotic can be added to the broth or agar plates in order to grow bacteria which have the gene insert conferring its resistance to that antibiotic. Following points are necessary to consider for optimal growth conditions: 1. In general, most bacteria grow well at 37C, but there are some strains which require growth temperatures between 25-30C. 2. It is ideal in broth cultures to fill the flask to ⅓ or less of the total flask volume for optimal aerobic growth. 3. Shaking speeds between 140-180 rpm are appropriate to ensure aeration and that the cells are surrounded by fresh media, and do not settle.

Get tips on using HES1 (D6P2U) Rabbit mAb #11988 to perform Immunohistochemistry Human - Hes1

Get tips on using 1ml NCL-L-CK20-561 to perform Immunohistochemistry Human - CK20

Get tips on using XcelGen Blood gDNA Mini Kit to perform Whole Genome Amplification Human

Get tips on using EpiTect Whole Bisulfitome Kit (25) to perform Whole Genome Amplification Human

Get tips on using gRNA_Cloning Vector to perform CRISPR Human - Repression HPV-16 E7

Get tips on using gRNA_Cloning Vector to perform CRISPR Human - Repression HPV-16 E6

Get tips on using EMD Millipore™ Chemicon™ CpGenome™ Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - HepG2 FHIT

Get tips on using EMD Millipore™ Chemicon™ CpGenome™ Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - Hep3B SFRP3