rna-isolation-purification-cells-immortalized-ntera-2

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Ion Total RNA-Seq Kit v2 to perform RNA sequencing Rat - PC12

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Rat - PC12

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Rat - A7R5

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Mouse - RAW264.7

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - mouse kidney tissue

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - mouse liver tissue

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - mouse adipose tissue

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Rat - Trigeminal ganglia tissue

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.