rna-isolation-purification-tissue-mouse-spleen

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Get tips on using Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit to perform ELISA Mouse - IL-1 beta

Products R&D Systems Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Live / Dead assay mammalian cells mouse bone marrow-derived macrophages

Get tips on using HES1 Mouse Monoclonal Antibody [Clone ID: OTI1B5] to perform Immunohistochemistry Human - Hes1

Products OriGene HES1 Mouse Monoclonal Antibody [Clone ID: OTI1B5]

Get tips on using Monoclonal Anti-TBP antibody produced in mouse to perform Western blotting TBP

Products Sigma-Aldrich Monoclonal Anti-TBP antibody produced in mouse

Get tips on using Monoclonal Anti-MUC5AC antibody produced in mouse to perform Western blotting MUC5AC

Products Merck Millipore Monoclonal Anti-MUC5AC antibody produced in mouse

Get tips on using Phospho-Tau (Ser396) (PHF13) Mouse mAb #9632 to perform Western blotting Tau

Products Cell Signaling Technology Phospho-Tau (Ser396) (PHF13) Mouse mAb #9632

Get tips on using MinElute PCR Purification Kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Products Qiagen MinElute PCR Purification Kit

Get tips on using Purelink PCR Purification Kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Products Thermo Fisher Scientific Purelink PCR Purification Kit

Get tips on using QIAquick PCR Purification Kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Products Qiagen QIAquick PCR Purification Kit

Get tips on using APC/Cyanine7 anti-mouse I-A/I-E Antibody to perform Flow cytometry Anti-bodies Mouse - MHCII

Products BioLegend APC/Cyanine7 anti-mouse I-A/I-E Antibody

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