Get tips on using PE Mouse Anti-Human CD44 to perform Flow cytometry Anti-bodies Human - CD44
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Get tips on using pEGFP-INHα to perform Protein Expression Eukaryotic cells - BHK cells INHα
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DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.
Get tips on using PE Mouse Anti-Human CD140a to perform Flow cytometry Anti-bodies Human - CD140/PDFGR2
Get tips on using PE Mouse Anti-Human CD184 to perform Flow cytometry Anti-bodies Human - CD184/CXCR4
Get tips on using APC Mouse Anti-Human CD184 to perform Flow cytometry Anti-bodies Human - CD184/CXCR4
Get tips on using FITC Mouse Anti-Human CD36 to perform Flow cytometry Anti-bodies Human - CD36/CB38
Get tips on using Purified Mouse Anti-Human CD36 to perform Flow cytometry Anti-bodies Human - CD36/CB38
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