siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1)

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Tissue - Rat Pineal gland

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Tissue - mouse lung tissue

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Tissue - mouse brain tissue

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Primary cells HUVEC

Get tips on using Cell Proliferation Reagent WST-1 to perform Cell cytotoxicity / Proliferation assay cell type - SMMC-7721, Huh7, Hep3B, 293T

Get tips on using F4/80 antibody | Cl:A3-1 to perform Flow cytometry Anti-bodies Mouse - F4/80

Get tips on using PE anti-mouse CD138 (Syndecan-1) Antibody to perform Flow cytometry Anti-bodies Mouse - CD138/Syndecan-1

Get tips on using Purified anti-mouse CD138 (Syndecan-1) Antibody to perform Flow cytometry Anti-bodies Mouse - CD138/Syndecan-1

Get tips on using CD317 (PDCA-1) Antibody, anti-mouse, APC to perform Flow cytometry Anti-bodies Mouse - CD317/mPDCA-1