rna-isolation-purification-cells-primary-mouse-ventricles

Get tips on using Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit to perform ELISA Mouse - IGF-I

Get tips on using EasySep™ Direct Human T Cell Isolation Kit to perform Cell Isolation Human T cells

Get tips on using EndoFree Plasmid Mega Kit (5) to perform DNA isolation / purification Plasmid purification

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit to perform ELISA Rat - OPN

Get tips on using Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit to perform ELISA Rat - ANGPT2

Get tips on using Purified anti-mouse/rat/human FOXP3 Antibody to perform Western blotting FOXP3

Get tips on using ON-TARGET plus Mouse Becn1 (56208) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - 4T1 BECN-1

Get tips on using Immun-Star Goat Anti-Mouse (GAM)-HRP Conjugate to perform Western blot Secondary Antibody - Goat Mouse Horseradish peroxidase

Get tips on using FITC Mouse Anti-Human CD51/CD61 to perform Flow cytometry Anti-bodies Human - CD51