siRNA / miRNA gene silencing Human 501 Mel and SK Mel 28 FANCD2

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using MethylEasy™ Xceed (ME002) to perform DNA methylation profiling Gene specific profiling - Rat whole pituitary glands PROP1

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray Gene expression arrays - Mouse dorsal skin Biotin

Get tips on using pET-28b-hBCO1 to perform Protein Expression Prokaryotic cells - E. coli human BCO1

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Human Kidney

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Human Liver

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Human Veins

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Human Tonsil

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Human Prostate