rna-isolation-purification-tissue-mouse-lung

Get tips on using Monoclonal Anti-MUC5AC antibody produced in mouse to perform Western blotting MUC5AC

Get tips on using Phospho-Tau (Ser396) (PHF13) Mouse mAb #9632 to perform Western blotting Tau

Plasmid isolation is an important technique in molecular biology or any kind of genetic editing. It involves amplifying plasmids overnight by transforming them into competent bacterial cells. The desired colonies of these bacteria can then be grown in shaker cultures, at appropriate shaking speed, oxygen availability and temperature. These liquid cultures can then be ultracentrifuged to pellet the bacteria, which are then used for plasmid isolation. The bacteria are first resuspended in a buffer, then lysed, neutralized, purified in a column, eluted, precipitated with ethanol and then resuspended. During plasmid isolation, it is important to lyse cells quickly because lysing bacteria for too long may lead to irreversible denaturing of the plasmid. Usually, alkaline lysis is used for isolation because it is a mild treatment. It isolates plasmid DNA and other cell components such as proteins by breaking cells apart with an alkaline solution. Precipitation removes the proteins, and the plasmid DNA recovers with alcohol precipitation. Resuspension and lysis buffers should be mixed thoroughly in order to prevent the DNA from breaking into smaller fragments. This is because broken gDNA can reanneal and remain in the solution, without binding to the column.

Get tips on using MinElute PCR Purification Kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Get tips on using Purelink PCR Purification Kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Get tips on using QIAquick PCR Purification Kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Get tips on using APC/Cyanine7 anti-mouse I-A/I-E Antibody to perform Flow cytometry Anti-bodies Mouse - MHCII

Get tips on using Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit to perform ELISA Mouse - TGF-beta 1

Get tips on using Brilliant Violet 650™ anti-mouse IFN-γ Antibody to perform Flow cytometry Anti-bodies Mouse - IFN-γ

Get tips on using M-PER™ Mammalian Protein Extraction Reagent to perform Protein isolation Tissue - Human aortic endothelial cells