rna-isolation-purification-tissue-mouse-mammary-glands

Get tips on using Monoclonal Anti-TBP antibody produced in mouse to perform Western blotting TBP

Get tips on using Monoclonal Anti-MUC5AC antibody produced in mouse to perform Western blotting MUC5AC

Get tips on using Phospho-Tau (Ser396) (PHF13) Mouse mAb #9632 to perform Western blotting Tau

Get tips on using MinElute PCR Purification Kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Get tips on using Purelink PCR Purification Kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Get tips on using QIAquick PCR Purification Kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Get tips on using APC/Cyanine7 anti-mouse I-A/I-E Antibody to perform Flow cytometry Anti-bodies Mouse - MHCII

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit to perform ELISA Mouse - TGF-beta 1

Get tips on using Brilliant Violet 650™ anti-mouse IFN-γ Antibody to perform Flow cytometry Anti-bodies Mouse - IFN-γ