DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.
Get tips on using PE anti-human CD114 (G-CSFR) Antibody to perform Flow cytometry Anti-bodies Human - CD114
Get tips on using PE Mouse Anti-Human CD26 Clone L272 to perform Flow cytometry Anti-bodies Human - CD26
Get tips on using PE Mouse Anti-Human CD30 Clone BerH8 to perform Flow cytometry Anti-bodies Human - CD30
Get tips on using Monoclonal Mouse Anti-Human Cytokeratin, Clone MNF116 to perform Flow cytometry Anti-bodies Human - Keratin
Get tips on using CD11b Antibody, anti-human, PE, REAfinity™ to perform Flow cytometry Anti-bodies Human - CD11b
Get tips on using FITC anti-human CD15 (SSEA-1) Antibody to perform Flow cytometry Anti-bodies Human - CD15
Get tips on using A2B5 Antibody, anti-human/mouse/rat, APC to perform Flow cytometry Anti-bodies Human - A2B5
Get tips on using Human PAI-1 PicoKine™ ELISA Kit to perform ELISA Human - Serpin E1/PAI-1
Get tips on using Human C-Reactive Protein/CRP DuoSet ELISA to perform ELISA Human - C-Reactive Protein/CRP
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment