siRNA / miRNA gene silencing Human Calu-3

Get tips on using Notch 1 Antibody (A-8): sc-376403 to perform Immunohistochemistry Human - Notch1

Get tips on using Anti-Villin antibody [3E5G11] - N-terminal (ab201989) to perform Immunohistochemistry Human - Villin

Get tips on using Mucin 16 Antibody (C-6): sc-365002 to perform Immunohistochemistry Human - CA125

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - MIA PaCa-2

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - MDA-MB-231

Get tips on using KAPA Stranded mRNA-Seq Kit to perform RNA sequencing Human - PBMCs

Get tips on using EMD Millipore™ Chemicon™ CpGenome™ Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - HepG2 FHIT

Get tips on using EMD Millipore™ Chemicon™ CpGenome™ Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - Hep3B SFRP3

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.