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TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Cellular assays TUNEL assay cell type Rat fibroblast-like synoviocytes

Get tips on using Cultrex® BME Cell Invasion Assay to perform Cell migration / Invasion cell type - T47D

Products Bio-Techne Cultrex® BME Cell Invasion Assay

Get tips on using Cultrex® BME Cell Invasion Assay to perform Cell migration / Invasion cell type - HepG2

Products Bio-Techne Cultrex® BME Cell Invasion Assay

Get tips on using Cultrex® BME Cell Invasion Assay to perform Cell migration / Invasion cell type - A549

Products Bio-Techne Cultrex® BME Cell Invasion Assay

Get tips on using Cultrex® BME Cell Invasion Assay to perform Cell migration / Invasion cell type - HUVEC

Products Bio-Techne Cultrex® BME Cell Invasion Assay

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Apoptosis assay cell type Caspase 3/7

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Apoptosis assay cell type SMMC-7721, HEPG2

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay MIA PaCa-2

Products Bio-Techne CometAssay Single Cell Gel Electrophoresis Assay

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay MDA-MB-231

Products Bio-Techne CometAssay Single Cell Gel Electrophoresis Assay

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay U-87 MG

Products Bio-Techne CometAssay Single Cell Gel Electrophoresis Assay

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