DNA methylation profiling Gene specific profiling TCP-1, BCPAP

Get tips on using Atg12 Antibody (Mouse Specific) #2011 to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using CREB-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - HUVEC CREB

Get tips on using MEK-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - NHLF MEK1

Get tips on using ERK 1 siRNA (h) to perform siRNA / miRNA gene silencing Human - NHLF ERK1

Get tips on using ENX-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - HaCaT EZH2

Get tips on using Flotillin-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - A2780 FLOT1

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using IRF-1 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - B16-F10 IRF1

Get tips on using Gβ 1 siRNA (h) to perform siRNA / miRNA gene silencing Human - T47-D GNB1

Get tips on using caveolin-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - BEAS-2B CAV1