Site Directed Mutagenesis (SDM) Rat Deletion H9C2

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using SignalSilence® NF-κB p65 siRNA I #6261 to perform siRNA / miRNA gene silencing Rat - H9c2 NF-κB RelA (p65)

Get tips on using Image-IT™ LIVE Green Reactive Oxygen Species Detection Kit, for microscopy to perform ROS assay cell type - H9c2 rat cardiomyocytes

Get tips on using lentiCRISPR v2 to perform CRISPR Rat - Deletion PC12 MMP9

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Rat - Deletion AR42J FICD

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Rat - Deletion AR42J Atg12

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Rat - Deletion BMSCs Wisp2

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Rat - Deletion PC12 Munc18

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Rat - Deletion INS-1 832/13 Ep300

Get tips on using MYH9 CRISPR/Cas9 KO Plasmid (h) to perform CRISPR Rat - Deletion PC12 myosin IIA (Myh9)