Site Directed Mutagenesis (SDM) Human Point mutation PC-3

Get tips on using lenti dCAS-VP64_Blast to perform CRISPR Human - Activation interferon-γ promoter

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using EpiTect ChIP OneDay Kit to perform ChIP Human - MIA PaCa-2

Get tips on using TruSeq Stranded Total RNA to perform RNA sequencing Human - MCF-7

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification Human - HEK 293

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification Human - THP 1

Get tips on using QuantiFluor® RNA System to perform RNA quantification Fuorimetric - human plasma

Get tips on using PI/RNase Staining Buffer to perform Cell cycle assay human - U20S

Get tips on using PI/RNase Staining Buffer to perform Cell cycle assay human - U87

Get tips on using APC BrdU Flow Kit to perform Cell cycle assay human - FaDu