rna-isolation-purification-cells-primary-mouse-dorsal-root-ganglion-neurons

Get tips on using Human/Mouse Active Caspase-3 Antibody to perform Western blotting Caspase-3

Get tips on using Brilliant Violet 510™ anti-mouse CD69 Antibody to perform Flow cytometry Anti-bodies Mouse - CD69

Get tips on using PerCP-Cy™5.5 Hamster Anti-Mouse CD69 to perform Flow cytometry Anti-bodies Mouse - CD69

Get tips on using PE-Cy™7 Hamster Anti-Mouse CD11c to perform Flow cytometry Anti-bodies Mouse - CD11c

Get tips on using Mouse PAI-1 total antigen assay ELISA kit to perform ELISA Mouse - Serpin E1/PAI-1

Get tips on using Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit to perform ELISA Mouse - IGF-I

Get tips on using EasySep™ Direct Human T Cell Isolation Kit to perform Cell Isolation Human T cells

Get tips on using EndoFree Plasmid Mega Kit (5) to perform DNA isolation / purification Plasmid purification

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit to perform ELISA Rat - OPN