siRNA / miRNA gene silencing Human CAL-27

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Bone

Get tips on using Hoechst 33258 to perform DNA quantification Human - MDA-MB-231

Get tips on using gRNA_Cloning Vector to perform CRISPR Human - Repression HPV-16 E7

Get tips on using gRNA_Cloning Vector to perform CRISPR Human - Repression HPV-16 E6

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion STING exon 5

Get tips on using Anti-vimentin to perform Immunohistochemistry Vimentin (3B4) - Mouse Human -NA-

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification Human - MCF10A

Get tips on using TTF1 Monoclonal Antibody (2F4D8) to perform Immunohistochemistry Human - TTF-1