siRNA / miRNA gene silencing Rat Brain endothelial cells

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Get tips on using Dulbecco’s Modified Eagle’s Medium - high glucose to perform Stem cell culture media Rat oligodendrocyte precursors (OPCs)

Products Sigma-Aldrich Dulbecco’s Modified Eagle’s Medium - high glucose

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - rat MSC

Products Cell Biolabs CytoSelect™ 24-Well Wound Healing Assay

Get tips on using Viability/Cytotoxicity Assay kit for Bacteria Live and Dead Cells to perform Live / Dead assay bacteria - Salmonella enterica

Products Biotium Viability/Cytotoxicity Assay kit for Bacteria Live and Dead Cells

DNA DNA isolation / purification Cells Immortalized cell lines H1 hESc

Proteins Protein expression and purification Mammalian cells HEK 293 ECD

Proteins Protein expression and purification Mammalian cells HEK 293 AT1R

Proteins Protein expression and purification Mammalian cells HEK 293 AT2R

Proteins Protein expression and purification Mammalian cells HEK 293 HER2

Proteins Protein expression and purification Mammalian cells HEK 293 EGFR

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Cells Immortalized cell lines HEK 293T

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