RNA sequencing Rat

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - human liver tissue

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - human kidney tissue

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - human brain tissue

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary human keratinocytes

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Mouse Hippocampus

Get tips on using miRcute miRNA Isolation Kit to perform RNA isolation / purification Cells - immortalized H1299

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - primary porcine coronary artery endothelial cells

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - primary porcine coronary artery endothelial cells

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Cells - primary human umbilical vein endothelial cells